Biopiles or windrows are an ex situ application of bioremediation where petroleum. Successful design and implementation of a bioremediation program for soil, for. Mar 03, 2017 key difference in situ vs ex situ bioremediation bioremediation is a term used in biotechnology to refer the process of cleaning the polluted areas using biological organisms such as microorganisms and plants. Insitu and exsitu bioremediation technologies p1 youtube. Ex situ bioremediation list of high impact articles. The following discussion is organized accordingly, with descriptions of bioremedia tion processes for.
Ex situ bioremediation occurs at a location separate from the contaminated site and involves the translocation of the contaminated material. Engineered approaches to in situ bioremediation of. Ex situ bioremediation, in which contaminated soil is excavated and treated elsewhere, is an alternative. It can also disrupt the site and requires a good deal of space. Bioremediation as a sustainable alternative to chemicalphysical processes is an attractive solution for soil decontamination and renewal of polluted sites. Lca of exsitu bioremediation of dieselcontaminated soil. Ex situ bioremediation ex situ bioremediation involves removing contaminated soils from the ground for treatment that can occur in another location either on or off site. Types of in situ bioremediation1 intrinsic in situ bioremediation. Ex situ bioremediation approaches include use of bioreactors, landfarming, and biopiles. Us epa engineering issue in situ and ex situ biodegradation. In situ bioremediation involves treating the contaminated material at the site, while ex situ involves the removal of the contaminated material to be treated elsewhere. Ex situ bioremediation is usually more successful than in situ bioremediation.
Available approaches of remediation and stabilisation of. Increased microbial activities through aeration, irrigation, and creation of biopiles. In situ bioremediation an overview sciencedirect topics. Insitu soil and groundwater bioremediation techniques and. Because it may not require as much equipment, labor, or energy as some. Ex situ techniques can be faster, easier to control, and used to treat a wider range of contaminants and soil types than in situ techniques. In situ techniques are defined as those that are ap plied to soil and groundwater at the site with minimal disturbance, whereas ex situ techniques are applied to at. Performance criteria, which also determine the choice of ex situ bioremediation techniques, have been described philp and atlas 2005. This document presents information at a level of detail intended to familiarize federal and state project. In situ bioremediationisb, which is the term used in this report for gaseous nutrient injection for in situ bioremediation, remedi ates soils and ground water contaminated with volatile organic compounds vocs both above and below the water table.
Various bioremediation techniques can be applied in situ or ex situ and under aerobic or anaerobic conditions. Ex situ bioremediation method for the treatment of groundwater. Also, several other technical and biological arrangements at the reclamation site are required. It has been used commercially for more than 20 years. Ex situ bioremediation an overview research journal of. Phytoremediation acknowledgements glossary bibliography biographical sketch summary a brief outline of the development of bioremediation technologies is presented. Due to the ex situ techniques used to decontaminate the soil, much of the soil cannot be used as filling or agricultural material. In situ bioremediation of groundwater has become one of the most widely used technologies for contaminated site treatment because of its relatively low cost, adaptability to site. The goal in evaluating in situ bioremediation is to assess whether the weight of evidence from tests such as those described above documents a convincing case for successful bioremediation.
To outline the process of application, processing, approval and inspection, for bioremediation facilities 3. A systematic approach to in situ bioremediation in groundwater, including decision trees on in situ bioremediation for nitrates, carbon tetrachloride, and perchlorate isb8 aug02 provides guidance for the systematic characterization, evaluation, and appropriate design and testing of isb for any biotreatable contaminant. In situ bioremediation in situ bioremediation is the clean up approach which directly involves the contact between microorganisms and the dissolved and sorbed contaminants for biotransformation bouwer and zehnder, 1993. Investigators cannot fully understand the details of whether and how bioremediation is occurring at a site. In situ bioremediation of chlorinated solvent contaminated groundwater pdf file epatio. In situ groundwater bioremediation pdf file epatio. In situ and ex situ biodegradation have been proven to be very effective methods for remediating organic contaminations from soil and aquifers. Introduction to in situ bioremediation of groundwater pdf 86 pp, 2 mb, 20.
Exsitu bioremediation is a controlled remediation strategy in which the. Ex situ bioremediation environmental biotechnology. The design process commonly includes bench and pilotscale treatability studies. These tests may be performed during the feasibility study or remedial design, are used to evaluate whether the proposed bioremediation remedy will be successful,and obtain important design criteria. Ex situ processes involve the removal of the contaminated media to a treatment area. Ex situ bioremediation can use bioreactors and added nutrients. Icscs, 2006, manual for biological remediation techniques, international. The methods applied for bioremediation are in situ remediation, use of filters and bioreactors as the three theoretical categories bonaventura et al. Fundamental principles of bioremediation an aid to the. Bioremediation has the advantage of using natural processes to clean up sites.
Typically, temperatures of 54 to 65 c must be maintained to properly compost soil contaminated with hazardous organic contaminants. Use of bioresources for remediation of soil pollution. Increasingly, in situ bioremediation is being heralded as a promising new alternative ground water cleanup technology. A general essay on bioremediation of contaminated soil. In situ is a term utilized within a variety of fields meaning on site and refers to the location of an event. In 2001, the government of newfoundland and labrador implemented an islandwide ban on the. Ex situ remediation refers to reactions performed outside the natural habitat of these organisms. Bioremediation technologies for soil composting addition of moisture and nutrients, regular mixing for aeration biopiles exsitu aeration of soil bioventing insitu aeration of soil land treatment application of organic materials to natural soils followed by irrigation and tilling. Bioremediation is the process of decontaminating polluted sites through the usage of either endogenous or external microorganism.
It is 18 often considered to be less advantageous than in situ remediation because the contamination is moved elsewhere and has the possibility to create significant. In situ and ex situ bioremediation technologies p2 duration. The use of organisms to reduce and transform contaminants into nontoxic substances is an environmentally friendly process which does not ne. Bioremediation is based on the basic principles of biotechnology and microbiology, with special focus in in situ with site biogeohydrochemical processes and engineering principles. Pdf hydrocarbon contamination represents an important environmental issue due to the large number of polluted sites. Potential advantages of in situ bioremediation methods include minimal site disruption, simultaneous.
Examples of ex situ processes include land treatment and. Ex situ bioremediation of soil polluted with oily waste. Bioremediation techniques involve using these mechanisms to reduce pollutant amounts and are dependent on pollutant aspects. There is a wide selection of techniques available for ex situ bioremediation based on the severity, nature, and geographical location of the contamination fig. In situ bioremediation is the clean up approach which directly involves the contact between microorganisms and the dissolved and sorbed contaminants for biotransformation bouwer and zehnder, 1993. Bioremediation strategies are often more beneficial than traditional strategies because it can be implemented in situ directly at the site of the contaminant with no need to transport the contaminated material. The optimization and control of microbial transformations of organic contaminants require the integration of many scientific and engineering disciplines. In situ bioremediation in situ bioremediation involves a direct approach for the microbial degradation of pollution soil, ground water. Bioremediation techniquesclassification based on site of. Ex situ bioremediation method for the treatment of groundwater contaminated with pahs. Difference between in situ and ex situ bioremediation.
Introduction bioremediation is the use of living organisms, primarily microorganisms, for the degradation of hazardous chemicals in soil sediments, water, or other contaminated materials into less toxic forms microorganisms metabolize the chemicals to produce carbon dioxide or methane, water and biomass. Aug 03, 2016 methods of bioremediation on the basis of removal and transportation of the wastes for the treatment, basically there are two methods. Bioventing similar to biostimulation but it involves venting of oxygen through soil to stimulate growth of natural or introduced microorganisms. Bioremediation, contaminants, monitoring and saturation received 16 march 2011. It can be expensive, mostly due to the cost of excavation and transportation of the soil. Ex situ bioremediation techniques are usually considered based on. In this the contaminated soil is excavated and removed from the site as completely as possible.
In addition, in situ bioremediation is more publicly acceptable than above ground technologies because it relies on natural processes to treat contaminants. There is more certainty about the uniformity of treatment because of the ability to homogenize, screen, and continuously mix the soil. Bioremediation is based on the basic principles of biotechnology and microbiology, with special focus in insitu with site biogeohydrochemical processes and engineering principles. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the. Biotransformation in the surface environment is a very complex process.
Bioremediation technologies can be generally classified as in situ or ex situ. It also suffers from costs associated with solid handling process e. Combined exsitu and insitu remediation of soil and. Composting is a controlled biological process by which organic contaminants e. Ex situ bioremediation describes a process where contaminated soil or water is removed from the environment by biological organisms. And, ex situ bioremediation means you would scoop up the contaminated soil or pump up the contaminated water and take it somewhere else for bioremediation. Click on a document title to view it online and print.
In situ occurs within the site of contamination 1 in situ bioremediation can further be categorized by the metabolism occurring, aerobic and anaerobic, and by the level of human involvement. Bioremediation may also be conducted in a bioreactor, in which the contaminated soil or sludge is slurried with water in a mixing tank or a lagoon. Or, rightclick on the link and save the document to your local drive. In situ and ex situ bioremediation technologies p2 youtube. Ex situ processes also include land farming, which involves spreading contaminated soils over a large area on which cropping or plantation can be made. Technologies can be generally classified as in situ or ex situ. In situ treatment of contaminated sediments pdf file epaerl. See a citizens guide to excavation of contaminated soil epa 542f12007.
Dec 29, 2011 types of in situ bioremediation1 intrinsic in situ bioremediation. Lca of exsitu bioremediation of dieselcontaminated soil 11 pp laurence toffoletto 1. File for an rsc site 2 250 tonnes of soil removed recovery system to be installed in late 2010early 2011 mna to evaluate residual hydrocarbons and chlorinated solvents in situ bioremediation or possibly isco to treat residual hydrocarbon in source area during or following free phase recovery. The soil can, however, be used for landscaping purposes. Insitu and exsitu bioremediation technologies p1 duration. Introduction to in situ bioremediation of groundwater pdf 86 pp, 2 mb, 20, 542r08. The in situ bioremediation isb documents address the systematic characterization, evaluation, and appropriate design and testing of isb for biotreatable contaminants. Introduction to in situ bioremediation of groundwater es3. Innovative in situ technologies permit biological treatment of contaminated water by means of. Ex situ bioremediation technology includes most of disadvantages and limitations. Bioremediation an overview 163 are imported to a contaminated site to enhance degradation we have process known as bioaugmentation. In situ and ex situ biodegradation technologies for remediation of.
Exsitu bioremediation can use bioreactors and added nutrients. There is a wide selection of techniques available for exsitu bioremediation based on the severity, nature, and geographical location of the contamination fig. The first commercial in situ bioremediation system was installed in 1972 to. Ex situ bioremediation of contaminated soils 2111 more relevant for bioreactors that can be operated and controlled as industrial bioprocesses, but with higher costs in comparison to simpler ex situ. In the use of a bioreactor, contaminated soil is mixed with water and nutrients and the mixture is agitated by a mechanical bioreactor to stimulate. Within the context of bioremediation, in situ indicates that the location of the bioremediation has occurred at the site of contamination without the. Exsitu bioremediation technique involves excavation and transportation of polluted soils to a safe and reliable place for efficient treatment. Bioremediation is the use of any organism metabolism to remove pollutants. If a contaminant was present, the groundwater would need to be pumped in order to remove it. You may need a pdf reader to view some of the files on this page. Dec 10, 2017 in situ and ex situ bioremediation technologies p1 duration.
Sometimes ex situ treatment requires for the contaminated groundwater to be bathed in nutrients. Exsitu bioremediation describes a process where contaminated soil or water is removed from the environment by biological organisms. In aerobic bioventing, air is typically injected into the subsurface to facilitate aerobic metabolism of hydrocarbons. Hindu college, nagercoil, tamil nadu, india key words. Ex situ anaerobic bioremediation ex situ bioremediation occurs after contaminated soils are excavated. A major advantage of ex situ bioremediation processes is that most of the decontaminated soil can be reused. New insights into bioremediation strategies for oil. In situ bioremediation environmental biotechnology. Environmental protection agency, office of solid waste and emergency response, washington, d. Bioremediation that can occur on its ownfactors effecting rate of intrinsicbioremediation. Ex situ bioremediation ex situ bioremediation involves removal of waste materials and their collection at a place to facilitate microbial degradation. Pdf a critical analysis of exsitu bioremediation technologies of.
In situ bioremediation is the application of biological treatment to the cleanup of hazardous chemicals present in the subsurface. Comparison of ex situ and in situ techniques for bioremediation of. A general essay on bioremediation of contaminated soil msu. Thus bioventing may complement biostimulation as well as bioaugmentation. It consists of placing the excavated materials into an above ground enclosure. Ex situ bioremediation technique involves excavation and transportation of polluted soils to a safe and reliable place for efficient treatment. Ex situ bioremediation list of high impact articles ppts. Trick to learn in situ on site and ex situ off site conservation. Introduction to in situ bioremediation of groundwater. Ex situ bioremediation ex situ bioremediation requires excavation. Bioremediation techniques are designed to break down contaminants via the stimulation of microorganisms which use the contamination as an energy source for development and growth. Ex situ bioremediation involves extraction of contaminated media to other location for treatment.